How Do You Spell ALLERGENS RADIOIMMUNOSORBENT ASSAY?

1. Allergens Radioimmunosorbent Assay (ARISA) is a laboratory technique used to measure the levels of specific allergens in a person's blood serum. It is a type of immunoassay that combines the principles of radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA). The ARISA test is primarily performed to identify and quantify allergens responsible for allergic reactions in individuals.

   The procedure begins by collecting a blood sample from the patient, which is then processed to obtain serum. This serum is then mixed with specific antibodies that can bind to the suspected allergens. The mixture is incubated to allow the formation of immune complexes between the allergens and antibodies. During this stage, a radiolabeled substance or an enzyme-linked conjugate is introduced, which specifically binds to the immune complexes.

   Following incubation, specific instruments are used to measure the radioactivity or enzyme activity associated with the immune complexes. These measurements are proportional to the amount of allergens present in the blood sample, providing quantitative results. Higher levels of radioactivity or enzyme activity indicate a higher concentration of the allergen in the sample, suggesting a stronger allergic response.

   ARISA is commonly used to diagnose allergies, especially in cases where symptoms cannot be easily correlated with specific allergens. It can detect a wide range of allergens, including pollen, dust mites, animal dander, insect venom, and certain food components. The results obtained from the ARISA test help healthcare providers in determining the most effective treatment options, such as immunotherapy or allergen avoidance strategies, for individuals affected by allergies.

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